This study provides an in-depth and accessible analysis on biodiversity as a human right to inform the European Parliament’s work on how the European Union’s external action can best contribute to a holistic and human rights-based approach aimed at stopping biodiversity loss and degradation. After a brief overview of empirical data regarding the impacts of biodiversity loss on human rights and the limitations of available sources, the study assesses the status and content of existing international obligations on biodiversity and human rights. The study then assesses existing initiatives’ (potential) legal and political impact at international and regional levels for the EU to address biodiversity and human rights in a mutually supportive manner, within a variety of multilateral fora. Additionally, the study assesses the EU’s (unilateral and bilateral) external action tools that have addressed or could address the human rights dimensions of biodiversity in the context of development, trade and other areas of international cooperation. It provides a series of recommendations on how the European Parliament and other EU institutions can support the development of a holistic and human rights-based approach to the conservation and sustainable use of biodiversity in external action, including as part of the fight against climate change.